Fig. 2

Reduced hypoxic conditions and increased intestinal permeability in florfenicol-treated chickens. A and B Expression levels of PPAR-γ and HIF-1α in chicken cecum (n = 6 chickens per group) treated with PBS or florfenicol. C The maintenance of cecal hypoxia was examined by detecting the binding of pimonidazole (scale bar = 100 µm). D and E Cecal Occludin and Muc2 gene expression were measured in chickens fasted for 24 h before oral FITC-dextran administration. F The amount of FITC in the serum was measured 7 h later to assess gut permeability. FFC, florfenicol. Results are presented as the mean ± SEM. Statistical differences were determined in a t-test; *P < 0.05, **P < 0.01, ***P < 0.001