Fig. 2
Effects of overexpressed GSTA2X, GSTA3, GSTT1L, GSTZ1-1, and GSTZ1-2 on the LDH release (A–E), ROS accumulation (F) and AFBO-GSH production (G) in LMH cell treated with AFB1. A–E Control, cells without any treatment; Control+AFB1, cells treated with 100 μg/L AFB1; pcDNA3.1(+), the cells transfected with pcDNA3.1(+) plasmid; pcDNA3.1(+) +AFB1, the cells transfected with pcDNA3.1(+) plasmid plus AFB1 treatment; GSTs, the cells transfected with different pcDNA3.1(+)-GST plasmid; GSTs+AFB1, the cells transfected with different pcDNA3.1(+)-GSTs plus AFB1 treatment. F Control, cells treated with 100 μg/L AFB1; pcDNA3.1(+), the cells transfected with pcDNA3.1(+) plasmid plus AFB1 treatment; GSTs, the cells transfected with different pcDNA3.1(+)-GSTs plus AFB1 treatment. G Control, cells without any treatment; pcDNA3.1(+), the cells transfected with pcDNA3.1(+) plasmid; AFB1, cells treated with 100 μg/L AFB1; pcDNA3.1(+) +AFB1, the cells transfected with pcDNA3.1(+) plasmid plus AFB1 treatment; GSTs+AFB1, the cells transfected with different pcDNA3.1(+)-GSTs plus AFB1 treatment. Values are expressed as mean ± SD (n = 6–8). a–eDifferent letters between groups represent significant differences, P < 0.05