Fig. 4

Results of metagenomic sequencing of the rumen bacteria function in the CON and Leu (n = 6). A Prediction of microbial functional differences based on KEGG database. B Comparisons of the abundance of CAZymes genes of rumen microbiomes by the Welch’s t-test in metagenomic sequencing. The differences are represented by the color of the group, “CON” means basal diet group; “Leu” means basal diet supplementation with 6.0 g L-Leu/100 kg BW/d group. ^*P < 0.05, ^**P < 0.01