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Table 3 Effects of L-Leu on ruminal free amino acid concentrations at 4 h post-feeding in beef cattle (n = 6)

From: Dietary L-leucine supplementation improves ruminal fermentation parameters and epithelium development in fattening Angus beef cattle

Item, µg/mL

Treatment

SEM1

P-value

CON

Leu

Methionine

1.02

1.35

0.127

0.222

Threonine

3.40

5.09

0.462

0.058

Valine

3.18

3.53

0.232

0.495

Isoleucine

1.90

2.97

0.297

0.064

Leucine

3.45

3.22

0.468

0.831

Phenylalanine

2.42a

3.64b

0.279

0.011

Lysine

5.97a

10.96b

1.266

0.034

Total EAA

21.35

30.79

2.772

0.085

Asparagine

2.85

4.17

0.429

0.129

Cystine

0.92a

0.55b

0.084

0.010

Serine

2.11

2.61

0.197

0.234

Glutamine

15.46

20.95

2.354

0.275

Glycine

1.49

1.86

0.137

0.190

Alanine

5.11

5.82

0.400

0.414

Tyrosine

2.56a

3.60b

0.261

0.033

Histidine

0.05

0.17

0.032

0.066

Arginine

0.79

0.67

0.132

0.692

Total NEAA

31.36

40.43

3.790

0.261

Total BCAA

8.53

9.73

0.860

0.530

Total AA

52.71

71.22

6.479

0.167

  1. CON Basal diet group, Leu Basal diet supplementation with 6.0 g L-Leu/100 kg BW/d group, BCAA Branched-chain amino acid, EAA Essential amino acid, NEAA Nonessential amino acid
  2. 1Standard error of the mean
  3. a,bDifferent superscript letters within the same row indicate significant differences (P < 0.05)