Fig. 1

Optimization of stable pTr-CDX2/luc-TEAD4/Rluc dual luciferase report cells for high-throughput screening. A Construction of a high-throughput screening cell model. B and C CDX2/TEAD4 gene promoter constructs of different lengths were cloned into pGL4.17 Luciferase reporter vector and transfected into pTr cells, followed by incubation for 48 h. The fold change in Luciferase activity of the cells transfected with each CDX2/TEAD4 promoter construct relative to that of the cells transfected with the promoter-less basic vector is shown. D and E Luciferase/Renilla activity in each cell clone (A1–C6). Data were expressed as means ± SEM. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, ^****P < 0.0001