Fig. 4

Validation of candidate amino acid system through in vitro experiments. A Effect of candidate amino acid system and #25 amino acid combination on CDX2 promoter expression. B Impact of candidate amino acid system and #25 amino acid combination on TEAD4 promoter expression. C Influence of candidate amino acid system and #25 amino acid combination on gene expression of CDX2, TEAD4, and GATA3. D and E Effects of candidate amino acid system and CDX2, TEAD4 interference on in vitro embryo adhesion rate. Data were presented as means ± SEM.^*P < 0.05, ^**P < 0.01, ^***P < 0.001, ^****P < 0.0001. ^a–cDifferent letters represented differences between groups. #25 represents the 25th amino acid combination medium