Fig. 5

Effect of candidate amino acid supplementation on transcriptome profiling of mice blastocysts. A Volcano plot displaying differentially expressed genes in the candidate amino acid system and control groups. B Gene Ontology (GO) enrichment analysis of differentially expressed genes (DEGs) in the candidate amino acid system and control groups. C Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis of DEGs in the candidate amino acid system and control groups. D Heatmap illustrating DEGs related to cell cycle regulation and differentiation. E Impact of candidate amino acid system supplementation on cell cycle regulation. F Impact of candidate amino acid system supplementation on cell cycle differentiation regulation. G Heatmap illustrating DEGs related to amino acid transporters in mouse blastocysts of the candidate amino acid system and control groups. H Impact of candidate amino acid system supplementation on amino acid transporter expression in pTr cells. I Influence of candidate amino acids supplementation on amino acid transporter expression in pig placenta. Data were presented as means ± SEM. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, ^****P < 0.0001