Fig. 1

In vitro inhibitory effects of usnic acid and tannic acid on coccidia, sporozoites, and Clostridium perfringens. A and D Impact of usnic acid and tannic acid on the apoptosis of coccidia and sporozoites, respectively, n = 6; B and E Effects of usnic acid and tannic acid on the mitochondrial membrane potential of coccidia and sporozoites, respectively, n = 6; C Morphology and fluorescence of coccidian oocysts observed under a laser confocal microscope; F The inhibitory effects of usnic acid and tannic acid on C. perfringens using the paper slide method; G The inhibition zone statistics, n = 9. NC: control group; DZ: 2 μmol/L diclazuril group; UA: 30 μmol/L usnic acid group; TA: 90 μmol/L tannic acid group; UTA: 15 μmol/L usnic acid + 45 μmol/L tannic acid group; PEN: 100 IU/mL penicillin group; UA1: the 3 mmol/L UA group; TA1: the 88 mmol/L TA group; UTA1: the 1.5 mmol/L UA + 44 mmol/L TA group. a–dDifferent letters indicate significant differences (P < 0.05)